| Autor: |
Schneede, Peter, Jelkmann, Wolfgang, Schramm, Uda, Fricke, Harald, Steinmetz, Manfred, Hofstetter, Alfons |
| Zdroj: |
Lasers in Medical Science; Mar1988, Vol. 3 Issue 1-4, p249-257, 9p |
| Abstrakt: |
The cellular mechanisms of action of low power lasers are only partially understood. Therefore, we have studied effects of helium-neon (He−Ne) laser irradiation in a cell culture model. The studies were performed with the permanent rat kidney cell line RK-L. By electron microscopy, significant ultrastructural changes of the cells were seen after He−Ne irradiation at 40 mW/cm for 4h (569 J/cm), including detritus-like formations of the cell organelles close to the nucleus. While such changes were not observed after 1 h irradiation (142 J/cm), the number of cells in mitosis increased under this condition. In addition, the rate of incorporation ofH-thymidine into DNA decreased transiently 6-9 h after 1 h He−Ne irradiation of the cells. The consumption of glucose was also lowered for 10 h after irradiation, while the production of lactate increased. Finally, the synthesis of prostaglandin E was reduced following irradiation. These results show that the He−Ne laser induces significant effects at the cellular level. In addition, our findings support the concept that the biological effects of low power laser treatment involve changes in the cell cycle. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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