| Autor: |
Lozano-Chaves, Elisa, Hernández-Artiga, Maria, Muñoz-Leyva, Antonio |
| Zdroj: |
Microchimica Acta; 1994, Vol. 116 Issue 1-3, p91-99, 9p |
| Abstrakt: |
A new spectrophotometric method for the determination of phosphate in urine is proposed, based on the ligand exchange extraction produced by phosphate solution at pH 7.5 when it is shaken with a solution of Fe(III) indole-2-carboxylate in iso-amyl alcohol. The organic layer decoloration is measured at 400 nm and the phosphate indirectly determined. The sensitivity of the method is 1.07 × 10 1 mol cm, the linear working range 1-15 × 10 mol/l of phosphate and the relative standard deviation 5%. It does not require previous treatment of the sample and it is free from interferences by other normal or pathological constituents of the urine samples. The results agreed closely with those obtained by using a Sigma Diagnostics procedure for inorganic phosphorus, based on the Fiske-SubbaRow method. The method does not require special instrumentation and is not expensive. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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