| Autor: |
Brotherick, Ian, Shenton, Brian, Cowan, William, Angus, Brian, Horne, Charles, Higgs, Michael, Lennard, Thomas |
| Zdroj: |
Cancer Immunology, Immunotherapy; May1995, Vol. 41 Issue 3, p137-145, 9p |
| Abstrakt: |
Quantification of c- erb B-2 and its relationship with other prognostic markers using flow cytometry has been examined. In this study a level for c- erb B-2 expression above which tumours are classified as positive by flow cytometry has been determined by employment of positive cut-off threshold levels. c- erb B-2 expression by both flow cytometry and immunohistochemistry was studied using the monoclonal antibody NCL-CBII. The relationship of c- erb B-2 quantification by flow cytometry was then compared with ploidy, axillary node status, tumour size and grade. Increased c- erb B-2 expression was seen using flow cytometry. Correlation between immunohistochemistry and flow-cytometry methods just failed to reach significance ( P=0.06). Immunohistochemistry revealed a significant relationship between c- erb B-2 expression and aneuploidy ( P=0.04). Cytokeratinpositive cells from 110 samples obtained from patients with breast cancer were assayed for DNA content and c- erb B-2 expression by flow cytometry. No correlation was seen between these parameters upon application of Mann Whitney analysis. However, examination of fluorescence thresholds showed a positive correlation between grade and c- erb B-2 expression at a level of more than 3200 molecules ( P≦0.03). At the level of 3600 molecules significance was increased ( P=0.004). These levels equated with between 15% and 19% of the samples being classified as c- erb B-2 positive. Application of these cut-off points showed no correlation between c- erb B-2 expression and ploidy, tumour size or axillary node status. Comparison of ploidy and grade showed a significant association ( P=0.0015), increased grade correlating with aneuploidy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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