| Abstrakt: |
Six ultraviolet-light(UV)-induced tumors of (BALB/c×C57BL/6)F (H-2) mouse origin were analyzed for the effector T cell subsets involved in tumor rejection the MHC class I to which cytolytic T lymphocytes (CTL) are restricted, and the effect of UV radiation on tumor rejection, to characterize their tumor-rejection antigens (TRA) recognized by CTL. All tumors were rejected in syngeneic normal mice but grew progressively in nude mice. CD8 T cells mediated the antitumor responses for all tumors and CD4 T cells could also do so for one tumor 6.1B. Each tumor induced potent CTL that recognized the specific TRA in preferential association with MHC class I haplotypes not from H-2 but from H-2; that is, K, D or L. Profiles of TRA expression on two tumors were obtained by the analyses of their antigen-loss variants. ♀1A codominantly expressed at least four distinct TRA associated with K, all of which induced CTL. On the other hand, UV♂ 1 had at least two distinct TRA, one of which, associated with K, exclusively induced CTL. However, in the absence of the dominant TRA, another TRA associated with L on R95C, a variant of UV♂, 1, induced CTL. Unlike other tumors, R95C grew progressively in short-term-UV-irradiated syngeneic mice. Nude mice reconstituted with a combination of CD4 T cells from short-term-UV-irradiated mice and CD8 T cells from normal mice did not reject R95C. An increase in the former T cell population led the reconstituted mice to reject the tumor. These findings suggest some functional defects of CD4 T cells rather than the generation of suppressor cells in short-term-UV-irradiated mice. The UV-induced tumors used in the present study provide a unique system for analyzing the preferential sorting of TRA as well as for elucidation of the TRA itself. [ABSTRACT FROM AUTHOR] |
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