| Autor: |
David, Hélène, Bade, Pascale, David, A., Savy, Colette, Demazy, Catherine, Cutsem, P. |
| Zdroj: |
Protoplasma; 1995, Vol. 186 Issue 3/4, p122-130, 9p |
| Abstrakt: |
Flax hypocotyl protoplasts were embedded in agarose and alginate beads. The pectin molecules of the formed colonies were observed in electron microscopy using 2F4 antibody specific of a calcium-induced supramolecular conformation of homopolygalacturonic acid. Little pectin, mostly methylesterified, was present in agarose-entrapped colonies. The regenerating cells immobilized in alginate secreted much higher amounts of methylesterified pectins in their walls. De-esterification of the pectins was clearly seen after 6 days of culture. These results illustrate the importance of the external matrix on wall differentiation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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