| Autor: |
Korohoda, W., Vöth, Monika, Bereiter-Hahn, J. |
| Zdroj: |
Protoplasma; 1992, Vol. 167 Issue 3/4, p169-174, 6p |
| Abstrakt: |
Human polymorphonuclear leucocytes and epitheliocytes isolated from tadpole tails of Xenopus laevis were used to observe the responses of cells to mechanical stimulation with a microneedle. Biphasic responses were observed: a retraction phase lasting 1-3 s was followed by an extension phase lasting 10-40s. Weak stimulation evoked a local response whilst on strong stimulation the whole cells rounded up. Spreading after induced rounding was at least one order of magnitude faster (it lasted less than 1-2min) than cell spreading after chemical dissociation of cell cultures. Local or extended loss of cell attachment to the substratum (observed with reflection interference contrast microscopy) preceded changes in cell morphology, visible with phase contrast microscopy. Repeated weak stimulation of one cell side induced extension and locomotion of the cell in this direction. The reported biphasic responses of cells to mechanical stimulation highlight the significance of exact timing when following any cell response to external stimuli. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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