| Abstrakt: |
We have successfully localized fungal actin for the first time using immuno-electron microscopy and hyphal tips of the rice blast pathogen Magnaporthe grisea. Following ultrarapid freezing, samples were processed in a novel substitution fluid of 10% acrolein in anhydrous ethanol and embedded in LR White resin. A monoclonal anti-actin antibody, previously shown to recognize M. grisea actin, bound specifically to filasomes concentrated in the peripheral cytoplasm of subapical regions, and to the core-region of the Spitzenkörper. [ABSTRACT FROM AUTHOR] |
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