Transgenic plants of ramie ( Boehmeria nivea Gaud.) obtained by Agrobacterium mediated transformation.

Autor: Dusi, Diva, Dubald, Manuel, Almeida, Elionor, Caldas, Linda, Gander, Eugen
Zdroj: Plant Cell Reports; Sep1993, Vol. 12 Issue 11, p625-628, 4p
Abstrakt: A regeneration and transformation protocol for ramie ( Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. Co-cultivation of leaf discs with Agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of expression of the GUS-marker gene. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index