Improved embryoid induction and green shoot regeneration from wheat anthers cultured in medium with maltose.

Autor: Orshinsky, Brian, McGregor, Linda, Johnson, Grace, Hucl, Pierre, Kartha, Kutty
Zdroj: Plant Cell Reports; Nov1990, Vol. 9 Issue 7, p365-369, 5p
Abstrakt: Anthers from spring wheat ( Triticum aestivum L.) genotypes, including six F hybrids, were cultured in a modified liquid N6 medium containing either sucrose or maltose. In every case, use of maltose resulted in greater microspore callus induction and green shoot regeneration than culture in sucrose-containing medium. Induction in maltose medium also allowed green shoots to be recovered from crosses that showed only a poor response in other media and from two genotypes that did not respond to modified N6 medium with sucrose. Replacement of sucrose with maltose generally resulted in microspores having a more embryogenic mode of development in which distinct embryoids often formed. The most responsive genotype produced over 200 green shoots/100 anthers when cultured in medium with maltose. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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