Expression of a chimaeric kanamycin resistance gene introduced into the wild soybean Glycine canescens using a cointegrate Ri plasmid vector.

Autor:	Rech, E., Golds, T., Husnain, T., Vainstein, M., Jones, B., Hammatt, N., Mulligan, B., Davey, M.
Zdroj:	Plant Cell Reports; May1989, Vol. 8 Issue 1, p33-36, 4p
Abstrakt:	Seedling hypocotyl explants of Glycine canescens were inoculated with Agrobacterium rhizogenes carrying a chimaeric NPTII gene cointegrated into the TL-DNA of pRiA4. Transformed roots produced shoots on B5 based medium with 10.0 mgl BAP, 0.05 mgl IBA and 50 μgml kanamycin. Cultured roots and regenerated plants expressed NPTII enzyme activity which was correlated with the presence of Ri TL-DNA and the structural sequence of the NPTII gene. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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