| Autor: |
Harris, Rita, Wright, Martha, Byrne, Michael, Varnum, James, Brightwell, Blanche, Schubert, Karel |
| Zdroj: |
Plant Cell Reports; Aug1988, Vol. 7 Issue 5, p337-340, 4p |
| Abstrakt: |
Protoplasts were isolated from anther-derived suspension cultures of commercial wheat ( Triticum aestivum L. cv. Chris). The protoplasts were released enzymatically and isolated by centrifugation on a sucrose cushion. The isolated protoplasts were initially cultured in a liquid medium in the dark. Numerous microcalli were produced under these conditions, some of which differentiated into globular embryos. Upon transfer to a solid medium and exposure to 16h/8h light/dark cycle, the protocalli proliferated and many of the somatic embryos matured. Complete plantlets were obtained and maintained in sterile culture. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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