| Autor: |
Menting, Sandra, Thai, Khoa T. D., Nga, Tran T. T., Phuong, Hoang L., Klatser, Paul, Wolthers, Katja C., Binh, Tran Q., de Vries, Peter J., Beld, Marcel |
| Předmět: |
|
| Zdroj: |
Advances in Virology; 2011, Vol. 2011, p1-9, 9p, 1 Diagram, 7 Charts, 1 Graph |
| Abstrakt: |
Dengue has become a global public health problem and a sensitive diagnostic test for early phase detection can be life saving. An internally controlled, generic real-time PCR was developed and validated by testing serial dilutions of a DENV positive control RNA in the presence of a fixed amount of IC with results showing a good linearity (R2 = 0.9967) and a LOD of at least 1.95 × 104 copies/mL. Application of the generic PCR on 136 patient samples revealed a sensitivity of 95.8% and specificity of 100%. A newly developed multiplex real-time PCR with serotype-specific probes allowed the serotyping of DENV for 80 out of 92 (87%) generic real-time PCR positive patients. Combined these real-time PCRs offer a convenient diagnostic tool for the sensitive and specific quantification of DENV in clinical specimens with the possibility for serotyping. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
