| Autor: |
Bisht, Shivani, Bisht, N., Bhandari, Snehlata |
| Zdroj: |
Physiology & Molecular Biology of Plants; Jan2012, Vol. 18 Issue 1, p89-93, 5p |
| Abstrakt: |
An ideal micropropagation method of Polygonatum verticillatum has been developed using stem disc explants. Multiple shoots were initiated from stem disc explants on Murashige and Skoog (MS) medium fortified with different concentrations (0.25-10.0 mgl) and combinations of cytokinins (BAP, Kn and TDZ) along with (0.5-1.0 mgl) auxins (NAA/IBA/IAA). 1.0 mgl BAP with 0.5 mgl NAA was found to be the most effective in producing maximum number of shoots. Regular subculturing of these in vitro multiple shoots induced profuse growth of lateral roots in the same medium. Individual shoots were excised and rooted in vitro on half strength MS medium with 1.0 mgl NAA. Regenerants were hardened in growth chamber with high humidity and showed a high rate of survival. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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