Assay of extracellular proteinases using a colorimetric collagen substrate for the differentiation of Serratia in the tribe Klebsielleae.

Autor: Edberg, S., Edberg, M.
Zdroj: Medical Microbiology & Immunology; Jul1985, Vol. 174 Issue 3, p115-118, 4p
Abstrakt: The gelatin test has been utilized for many years as a characteristic to separate the genus Serratia from other members of the tribe Klebsielleae. Gelatin is a large protein matrix that cannot diffuse into bacterial cells. Microbes that attack gelatin do so by producing extracellular proteinases. The measurement of gelatinase has suffered from the lack of a defineable endpoint and the inability to quantitate the enzyme. A method was developed utilizing an azodye-labelled collagen substrate that could measure the extracellular proteinase of Serratia. The test was easy to perform, inexpensive, and potentially quantifiable. The azodye test corresponded completely with the gelatinase tests. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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