Electron-microscopic identification of infectious particles of lymphocytic choriomeningitis.

Autor: Blechschmidt, M., Thomssen, R.
Zdroj: Medical Microbiology & Immunology; Dec1976, Vol. 162 Issue 3/4, p193-199, 7p
Abstrakt: LCM virus, strain WE-grown on L cells- and labeled withH-uridine was centrifuged to equilibrium in a sucrose density gradient and examined in fractions for infectivity, incorporated radioactivity, and electron-microscopic features. The peak of infectivity is congruent with the one of radioactivity (density=1.17 g/ml). LCM virus specificity of the radioactive peak was proved by precipitation of the radioactivity with anti-LCM virus antiserum. The peak fractions showed an abundance of 106±14 nm (1 s) particles. They could be agglutinated with specific anti-LCM virus antiserum but not with antiserum directed against the histocompatibility (H-2) antigens of L cells. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index