5′-flanking DNA of the human insulin receptor gene and long terminal repeat of mouse mammary tumour virus bind to the same nuclear protein(s).

Autor: Iwama, N., Saito, Y., Nomura, M., Imano, E., Watarai, T., Yamasaki, Y., Kawamori, R., Kamada, T.
Zdroj: Diabetologia; Dec1989, Vol. 32 Issue 12, p877-880, 4p
Abstrakt: The interaction of nuclear protein extracted from rat liver and 5′-flanking DNA of the human insulin receptor gene was investigated with the aid of gel mobility shift analysis. When 5′-flanking DNA (-1255/-1206 or -385/-345 base pairs) was incubated with nuclear protein, two or three P-DNA species (protein binding DNA fragment(s) and free DNA fragment) were detected. These bands did not disappear in spite of increasing amounts of synthetic poly(dI-dC), showing that nuclear protein binds specifically to 5′-flanking DNA of the insulin receptor gene. Increasing amounts of long terminal repeat of mouse mammary tumour virus resulted in a reciprocal decrease in nuclear protein binding to 5′-flanking DNA of insulin receptor gene. These results suggest that 5′-flanking DNA of insulin receptor gene binds to the same nuclear protein to which long terminal repeat of mouse mammary tumour binds. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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