| Autor: |
Hellman, B., Idahl, L., Sehlin, J., Täljedal, I. |
| Zdroj: |
Diabetologia; Dec1975, Vol. 11 Issue 6, p495-500, 6p |
| Abstrakt: |
When equilibrated with O-CO (95:5), pancreatic islets of non-inbred ob/ob-mice exhibited a sigmoidal dependence ofHO production on D-(5-H)-glucose concentration; the rate was most sensitive to changes of glucose concentration around 5mM and tended to be maximum above about 15 mM glucose.HO production from more than 5 mM D-(5-H)-glucose was about twice as fast as the production ofCO from equimolar D-(U-C)-glucose. Islets equilibrated with N-CO (95:5) did not exhibit a sigmoidal dose-response curve forHO production, the process being inhibited by anoxia at glucose concentrations above 5mM. Pieces of exocrine pancreas had a slower aerobicHO production than the islets and showed a clear enhancement of the process during anoxia. In comparison with oxygenated islets, anoxic islets exhibited decreased concentrations of glucose-6-phosphate and increased concentrations of fructose-l,6-diphosphate. The concomitant inhibition of glycolytic flux may be due to a low lactate dehydrogenase activity in islets yielding a slow reoxidation of NADH and a slow phosphoglyceraldehyde oxidation under anaerobic conditions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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