| Autor: |
Nakayama, T., Hashimoto, Y., Kaneko, Y., Yoshida, M., Beppu, T., Ohmi, K., Yamashita, S., Nonomura, Y., Kurokawa, K. |
| Zdroj: |
Experientia; Oct1993, Vol. 49 Issue 10, p876-880, 5p |
| Abstrakt: |
The administration of 200 nM K252a to HuH7 suppressed the proliferation of the cells almost completely. The uptake of [H]thymidine was inhibited, and flow cytometry revealed only one peak at 2C on day 3 after treatment with 100 nM K252a. The expression of proto-oncogene c-myc was not reduced. Despite the blockage at G1, both the size of the cells and the amount of cell protein had increased by 4 times by day 3 after treatment with K252a, while the cells secreted albumin and α-fetoprotein into the medium as usual. These results show that K252a can increase the cell size of HuH7 without losing its function by blocking the cell cycle at G1 phase. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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