Purification and characterization of pectinesterase produced by a strain of Aspergillus niger.

Autor: Maldonado, María, Strasser de Saad, Ana, Callieri, Danley
Zdroj: Current Microbiology; Apr1994, Vol. 28 Issue 4, p193-196, 4p
Abstrakt: After an 88-fold purification, pectinesterase produced by a strain of Aspergillus niger, isolated from rotten lemons, showed the following main characteristics: maximum activity at 45°C, pH 5; Km, with pectin as substrate, 1.01 mg/L; ΔG, 4750 Cal/mol. Polygalacturonic acid and methanol acted as competitive and non-competitive inhibitors, respectively. The activity of the enzyme was impaired by MgCl and stimulated by NaCl. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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