Circadian rhythm mutants of the prokaryotic Synechococcus RF-1.

Autor: Huang, Tan-Chi, Wang, Sheng-Tsann, Grobbelaar, Nathanaël
Zdroj: Current Microbiology; Nov1993, Vol. 27 Issue 5, p249-254, 6p
Abstrakt: Synechococcus RF-1 established circadian rhythms in nitrogen fixation and leucine uptake when growing in a diurnal light/dark regimen. The rhythms persisted in subsequent uniform light/light conditions. In order to analyze the circadian rhythm at the genetic level, mutants were induced by N-methyl-N′-nitro-N-nitrosoguanidine and then isolated by procedures with the circadian nitrogen-fixing rhythm as a selecton marker. Characterization of the mutants with respect to the circadian rhythm indicated that some mutants were abnormal only in the nitrogen-fixing rhythm, while some simultaneously lost the ability to establish the nitrogen-fixing and leucine-uptake rhythms. The physiological properties of the circadian rhythm were compared. The genetic potential of the mutants that were abnormal in both rhythms is emphasized. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index