| Autor: |
Chang, Chung, Garnier, Monique, Zreik, Leyla, Rossetti, Victoria, Bové, Joseph |
| Zdroj: |
Current Microbiology; Sep1993, Vol. 27 Issue 3, p137-142, 6p |
| Abstrakt: |
A xylem-limited bacterium resembling Xylella fastidiosa has been shown previously by electron mmcroscopy to be associated with citrus variegated chlorosis (CVC), a new disease of sweet organe tress in Brazil. A bacterium was consistently cultured from plant tissues from CVC twigs of sweet orange trees but not from tissues of healthy trees on several cell-free media known to support the growth of Xylella fastidiosa. Bacterial colonies typical of X. fastidiosa became visible on PW, CS20, and PD2 agar media after 5 and 7-10 days of incubation, respectively. The cells of the CVC bacterium were rod-shaped, 1.4-3 μm in length, and 0.2-0.4 μm in diameter, with rippled walls. An antiserum against an isolate (8.1.b) of the bacterium gave strong positive reactions to double-antibody-sandwich (DAS), enzyme-linked immunosorbent assay (ELISA) with other cultured isolates from CVC citrus, as well as with several type strains of X. fastidiosa. This result indicates that the CVC bacterium is a strain of X. fastidiosa. ELISA was also highly positive with all leaves tested from CVC-affected shoots. Leaves from symptomless tress reacted negatively. Sweet organe seedlings inoculated with a pure culture of the CVC bacterium supported multiplication of the bacterium, which became systemic with 6 months after inoculation and could be reisolated from the inoculated seedlings. Symptoms characteristic of CVC developed 9 months post inoculation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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