| Autor: |
Hayashi, Sachio, Cho, Tamaki, Kaminishi, Hidenori, Hagihara, Yoshisato, Watanabe, Kenji |
| Zdroj: |
Current Microbiology; Jan1987, Vol. 15 Issue 1, p41-44, 4p |
| Abstrakt: |
The metabolism of l-proline to N-acetyl- d-glucosamine (GlcNAc) during germ tube formation of Candida albicans (C. albicans) ATCC 1002 was studied. In uptake experiments, 6.9 nmol of l-[C]proline were taken up by 1×10 cells during 3 h of incubation at 37°C. The percentage of germ tube formation was 94 under the same condition. The presence of GlcNAc reduced the uptake of l-proline to 3.0 nmol. The percentage of germ tube formation was 95 in the presence and absence of GlcNAc. The [H]GlcNAc uptake was 3.0 nmol and was constant whether l-proline was present or not. After the preparation of a chitin fraction from germ tubes that were labeled with l-[C]proline, the radioactivity from l-proline was detected in the glucosamine (GlcN) fraction by thin-layer chromatography (TLC). The metabolism of l-proline to GlcNAc in chitin during germ tube formation was confirmed in this experiment. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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