Formation and regeneration of protoplasts of wild-type Neurospora crassa.

Autor: Selitrennikoff, Claude, Bloomfield, Elizabeth
Zdroj: Current Microbiology; Mar1984, Vol. 11 Issue 2, p113-117, 5p
Abstrakt: Trichoderma reesei was grown using purified cell walls of Neurospora crassa as a primary source of carbon. The resulting culture medium contained an undefined mixture of N. crassa cell-wall digesting enzymes. Protoplasts (cell lacking wall) were formed when young N. crassa hyphae were treated with Trichoderma mixture. The vast majority of protoplasts resynthesized cell-wall material when washed free of cell-wall digesting enzyme; of these, about 40% regenerated a mycelium. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index