| Autor: |
Albert, Françoise, Buferne, Michel, Boyer, Claude, SchmittVerhulst, Anne-Marie |
| Zdroj: |
Immunogenetics; 1982, Vol. 16 Issue 6, p533-549, 17p |
| Abstrakt: |
To study the interactions between T cells and class I MHC products, we developed in vitro a T-cell line reactive to H-2K stimulating cells and derived T-cell clones from it. Although the T-cell line could proliferate in the absence of exogeneous T-cell growth factors when stimulated with H-2K spleen cells, each of the derived T-cell clones required both H-2K stimulating cells and an external source of T-cell growth factor for its propagation. Each of the T-cell clones was also cytolysic for H-2K target cells. Such T-cell clones allowed the comparison of the antigenic requirements for proliferation and cytolysis. By using H-2K mutant mice, we found that while the original anti-H-2K T-cell line reacted with each of the six mutants tested, the individual T-cell clones could be distinguished in terms of their reactivity pattern. Similar fine specificity patterns were found when H-2K mutant cells were used as stimulating or target cells for any given T-cell clone. Each of the three monoclonal H-2K-specific antibodies reacting with different epitopes of the H-2K molecule totally inhibited H-2K-induced proliferation and lysis by the T-cell clones. Further blocking studies involved use of Fab antibody fragments and definition of their reactivity on cells from the H-2K mutants. We concluded that: (1) blocking with a monoclonal antibody does not prove identity of alloantigens recognized by the T-cells and the antibody; (2) a monoclonal antibody could either block or not block H-2K-CTL interactions depending on structural variations of the H-2K molecule not affecting the CTL-H-2K functional interaction; (3) blocking one type of H-2K-T-cell interaction (induction of proliferation) always affects the other type (cytolysis). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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