Identification of an H-2K-related molecule by molecular cloning.

Autor: Reyes, Antonio, Johnson, Merrie, Schöld, Monica, Ito, Hirataka, Ike, Yoshimasa, Morin, Christophe, Itakura, Keiichi, Wallace, R.
Zdroj: Immunogenetics; 1981, Vol. 14 Issue 5, p383-392, 10p
Abstrakt: Based on the published amino-acid sequence of H-2K, we synthesized a mixture of eight 16-base long oligodeoxyribonucleotides representing all possible coding sequences for residues 51-56 (Trp-Met-Glu-Gln-Glu-Gly). The hexadecanucleotide mixture was used as a probe to screen recombinant DNA clones constructed from cytoplasmic PolyA RNA isolated from the murine thymoma cell line EL4 ( b haplotype). Of the 30 000 independent clones screened, one clone was found to hybridize with the probe. DNA sequence analysis showed that the cDNA clone was derived from a portion of an H-2K-related mRNA. The clone encodes a protein sequence identical with a region of H-2K in 42 consecutive residues (50 through 91). The sequence then diverges from the H-2K sequence and, after a single Glu codon, a termination codon is encountered. It is possible that this mRNA codes for a small 92 amino-acid protein with a sequence identical (except for a carboxy-terminal Glu residue) with the amino terminus of H-2K It is further speculated that this mRNA is coded for by the H-2K gene and differs from the H-2K mRNA in the pattern of posttranscriptional splicing. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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