| Autor: |
Kitanaka, Jun-ichi, Hamano, Takako, Gotoh, Masayuki, Hashimoto, Hitoshi, Baba, Akemichi |
| Zdroj: |
Neurochemical Research; Dec1994, Vol. 19 Issue 12, p1545-1550, 6p |
| Abstrakt: |
Effect of tunicamycin, an inhibitor of N-linked glycosylation, on prostaglandin (PG) F-stimulated phosphoinositide (PI) hydrolysis was examined in cultured rat astrocytes. Pretreatment of cultured astrocytes with tunicamycin (25-250 ng/ml) inhibited subsequent PGF (1 μM)-stimulated PI hydrolysis in concentration- and time-dependent manners. The inhibition completely recovered after removal of tunicamycin and re-incubation for 12 h. Tunicamycin pretreatment (100 ng/ml for 12 h) significantly blocked [S]methionine incorporation into cultured astrocytes, but cell viability was not affected under the condition. Inhibitors of processing of N-linked sugar chains such as bromoconduritol, 1-deoxymannojirimycin, and swainsonine had no effect on PI response to PGF. These observations suggest that PGF receptor is N-linked glycosylated. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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