Autor: |
Zanakis, M., Eskin, B., Ingoglia, N. |
Zdroj: |
Neurochemical Research; Feb1984, Vol. 9 Issue 2, p249-262, 14p |
Abstrakt: |
This study reports that 4S RNA present in regenerating optic axons of goldfish is likely to be transfer RNA. Evidence is also presented which indicates that this transfer RNA is similar to transfer RNA found in tectal cells and that its aminocylation is likely to occur both in retinal ganglion cells prior to axonal transport as well as in the axon itself. Fish with regenerating optic nerves received intraocular injections of [H]uridine followed 4 days later by intracranial injections of [C]uridine. Radioactive tectal 4S RNA was isolated 6 days after [H]uridine injections and chromatographed by BD cellulose chromatography. Optical density as well as radioactivity profiles for both [C]4S RNA (from tectal cells) and [H]4S RNA (90% of which originated from regenerating optic axons) were found to be similar to E. coli transfer RNA optical density profiles, indicating that the intra-axonal 4S RNA is likely to be transfer RNA. Moreover, comparisons ofH/C suggest that intra-axonal and cellular 4S RNAs are composed of similar species of transfer RNA. Results of other experiments indicated that aminoacylation of axonally transported tRNA occurs both in the retina and in optic axons subsequent to axonal transport. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
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