| Autor: |
Wittek, Alec E., Phelan, Michael A., Wells, Martha A., Vujcic, Luba K., Epstein, Jay S., Lane, H. Clifford, Quinnan Jr., Gerald V., Wittek, A E, Phelan, M A, Wells, M A, Vujcic, L K, Epstein, J S, Lane, H C, Quinnan, G V Jr |
| Předmět: |
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| Zdroj: |
Annals of Internal Medicine; Sep87, Vol. 107 Issue 3, p286-292, 7p |
| Abstrakt: |
A sensitive enzyme immunoassay was developed for detecting human immunodeficiency virus (HIV) core antigen. Assay sensitivity was 3.67 pmol/L of purified HIV core protein, and 1 or 100 in-vitro infectious units/mL of HIV in purified virus preparations or cell culture supernatants, respectively. Enzyme immunoassay sensitivity exceeded that of reverse transcriptase assay by 1000-fold. Core antigen was detected in whole plasma from 41% of symptomatic subjects and 13% of asymptomatic subjects seropositive for HIV. After plasma fractionation, antigenemia was found in 60% of symptomatic subjects and in 33% of asymptomatic subjects seropositive for HIV. Fifty-seven percent of samples from which HIV could be isolated in lymphocyte culture had detectable quantities of core antigen in plasma. However, at least 87% of samples with measurable antigen in plasma had HIV isolated from lymphocyte cultures. Antigenemia was associated with reduced T-cell number and symptomatic disease, and may be a useful marker for disease progression. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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