| Autor: |
Zhao Wei, Hao Yong-ming, Zou De-rong, Zhang Xiu-li, Lu Jia-yu, Hua Li, Cao Cun-hua |
| Předmět: |
|
| Zdroj: |
Shanghai Journal of Stomatology; Oct2011, Vol. 20 Issue 5, p464-469, 6p |
| Abstrakt: |
PURPOSE: To observe the biocompatibility and ectopic bone-like tissue formation of stem cells from goat deciduous teeth (SGDs) with porous calcium phosphate cement (pCPC). METHODS: The expression of STRO-1 on SGDs was measured with flow cytometry (FCM); the 4th passage SGDs were cultured in induced-mineralization medium in vitro for 7 days. Combined with pCPC , the adhesion and growth of the compounds were observed with scanning electron microscopy (SEM) ; the ectopic bone --like tissue formation was observed 8 weeks after the compounds implanted subcutaneously into the nude mice. RESULTS: On the third day of SGDs compounded with pCPC, SEM verified that the cells adhered closely and tightly with pCPC, protruded pseudopods and secreted matrix. 8 weeks after the compounds implanted in ectopic sites, HE staining confirmed the formation of bone-like tissue; Immunohistochemistry showed the strongly positive expression of OCN protein in the implanted materials. CONCLUSIONS: SGDs may differentiate into osteoblast and are potential to induce bone matrix formation; combined with pCPC, the compounds may generate bone--like tissue. Supported by Research Fund of Science and Technology Commission of Shanghai Municipality (09JC1411700), Collaborative Projects of Development and Research on Basic Science from Shanghai Institute of Stomatology (S30206-KF09) and Research Program of Shanghai Jiao Tong University School of Medicine (09XJ21030). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
