| Autor: |
Kobayashi, Masato, Mori, Tetsuya, Kiyono, Yasushi, Tiwari, Vijay Narayan, Maruyama, Rikiya, Kawai, Keiichi, Okazawa, Hidehiko |
| Předmět: |
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| Zdroj: |
Journal of Cerebral Blood Flow & Metabolism; Jan2012, Vol. 32 Issue 1, p33-40, 8p |
| Abstrakt: |
To develop a less-stressful and simple method for measurement of the cerebral metabolic rate of oxygen (CMRO2) in small animals, the steady-state method was applied to injectable 15O2-PET (15O2-positron emission tomography) using hemoglobin-containing vesicles (15O2-HbV). Ten normal rats and 10 with middle cerebral arterial occlusion (MCAO) were studied using a small animal PET scanner. A series of 15O-PET scans with C15O-labeled HbV, H215O, and 15O2-HbV were performed with 10 to 15 minutes intervals to measure cerebral blood volume (CBV), cerebral blood flow (CBF), and CMRO2. Positron emission tomography scans were started with a tracer injection using a multiprogramming syringe pump, which provides a slowly increasing injection volume to achieve steady-state radioactivity for H215O and 15O2-HbV scans. The radioactivity concentration of 15O rapidly achieved equilibrium in the blood and whole brain at about 2 minutes after H215O and 15O2-HbV administration, which was stable during the scans. The whole brain mean values of CBF, CBV, and CMRO2 were 54.3±2.0 mL per 100 g per minute, 4.9±0.4 mL/100 g, and 2.8±0.2 μmoL per g per minute (6.2±0.4 mL per 100 g per minute) in the normal rats, respectively. In the MCAO model rats, all hemodynamic parameters of the infarction area on the occlusion side significantly decreased. The steady-state method with 15O-labeled HbV is simple and useful to analyze hemodynamic changes in studies with model animals. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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