| Autor: |
Rangel, Thaiz Batista Azevedo, Assreuy, Ana Maria Sampaio, Pires, Alana de Freitas, de Carvalho, Amanda Uliana, Benevides, Raquel Guimarães, Simões, Rafael da Conceição, da Silva, Helton Colares, Bezerra, Maria Júlia Barbosa, do Nascimento, Antonia Samia Fernandes, do Nascimento, Kyria Santiago, Nagano, Celso Shiniti, Sampaio, Alexandre Holanda, Delatorre, Plínio, da Rocha, Bruno Anderson Matias, Fernandes, Patricia Machado Bueno, Cavada, Benildo Sousa |
| Předmět: |
|
| Zdroj: |
Molecules; Jun2011, Vol. 16 Issue 6, p5087-5103, 17p, 1 Black and White Photograph, 2 Diagrams, 3 Charts, 4 Graphs |
| Abstrakt: |
DwL, a lectin extracted from the seeds of Dioclea wilsonii, is a metalloprotein with strong agglutinating activity against rabbit and ABO erythrocytes, inhibited by glucose and mannose. DwL was purified by affinity chromatography on a Sephadex G-50 column and ion exchange chromatography on a HiTrap SP XL column. SDS-PAGE revealed three electrophoretic bands corresponding to the α (25,634 ± 2 Da), β (12,873 ± 2 Da) and γ (12,779 ± 2 Da) chains. Protein sequencing was done by Tandem Mass Spectrometry. The primary sequence featured 237 amino acids and was highly homologous to other reported Diocleinae lectins. A complete X-ray dataset was collected at 2.0 Å for X-Man-complexed DWL crystals produced by the vapor diffusion method. The crystals were orthorhombic and belonged to the space group I222, with the unit-cell parameters a = 59.6, b = 67.9 and c = 109.0 Å. DWL differed in potency from other ConA-like lectins and was found to induce neutrophil migration in rats, making it particularly useful in structural/functional studies of this class of proteins. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
