Storekeeper defines a new class of plant-specific DNA-binding proteins and is a putative regulator of patatin expression.

Autor: Zourelidou, Melina, de Torres-Zabala, Marta, Smith, Caroline, Bevan, Michael W
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Zdroj: Plant Journal; May2002, Vol. 30 Issue 4, p489-497, 9p
Abstrakt: Summary The expression of class I patatin genes is restricted to potato tubers but can be induced in other tissues by exogenous sucrose. Here we show that tuber-specific and sucrose-inducible gene expression is reduced in transgenic potato plants by mutations in a conserved 10 base pair motif within the B-box of the patatin promoter. In a southwestern screen, we have isolated a novel DNA-binding protein designated Storekeeper (STK) that specifically recognises the B-box motif in vitro . Gel shift experiments with an STK-specific antibody suggest that STK is the B-box binding protein found in tuber nuclei. We propose that STK, the defining member of a new class of DNA binding proteins, regulates patatin expression in potato tubers via the B-box motif. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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