| Autor: |
Kim, Wonyong, Park, Hee Kuk, Thanh, Hien Dang, Lee, Bo-Young, Shin, Jong Wook, Shin, Hyoung-Shik |
| Předmět: |
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| Zdroj: |
FEMS Microbiology Letters; Dec2011, Vol. 325 Issue 1, p77-84, 8p |
| Abstrakt: |
L actococcus garvieae, the pathogenic species in the genus L actococcus, is recognized as an emerging pathogen in fish, animals, and humans. Despite the widespread distribution and emerging clinical significance of L . garvieae, little is known about the genomic content of this microorganism. Suppression subtractive hybridization was performed to identify the genomic differences between L . garvieae and L actococcus lactis ssp. lactis, its closest phylogenetic neighbor, and the type species of the genus L actococcus. Twenty-seven clones were specific to L . garvieae and were highly different from L actococcus lactis in their nucleotide and protein sequences. Lactococcus garvieae primer sets were subsequently designed for two of these clones corresponding to a pyrH gene and a novel DNA signature for application in the specific detection of L . garvieae. The primer specificities were evaluated relative to three previously described 16 S r RNA gene-targeted methods using 32 L actococcus and closely related strains. Both newly designed primer sets were highly specific to L . garvieae and performed better than did the existing primers. Our findings may be useful for developing more stable and accurate tools for the discrimination of L . garvieae from other closely related species. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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