| Autor: |
Sage, D., Salin, P., Alcaraz, G., Castets, F., Giraud, P., Crest, M., Mazet, B., Clerc, N. |
| Zdroj: |
Journal of Comparative Neurology; Oct2007, Vol. 504 Issue 4, p363-378, 16p |
| Abstrakt: |
The types of sodium channels that are expressed by neurons shape the rising phase of action potentials and influence patterns of action potential discharge. With regard to the enteric nervous system (ENS), there is uncertainty about which channels are expressed, and in particular it is unknown whether Nav1.7 is present. We designed specific probes for the guinea pig Nav1.7 α subunit as well as for the other tetrodotoxin (TTX)-sensitive α subunits (Nav1.1, Nav1.2, Nav1.3, and Nav1.6) in order to perform in situ hybridization (ISH) histochemistry on guinea pig myenteric ganglia. We established that only Nav1.7 mRNA and Nav1.3 mRNA are expressed in these ganglia. The ISH signal for Nav1.7 transcripts was found in seemingly all the myenteric neurons. The expression of the Nav1.3 α subunit was confirmed by immunohistochemistry in a large proportion (62%) of the myenteric neuron population. This population included enteric sensory neurons. Nav1.6 immunoreactivity, absent from myenteric neurons, was detected in glial cells only when a high anti-Nav1.6 antibody concentration was used. This suggests that the Nav1.6 α subunit and mRNA are present only at low levels, which is consistent with the fact that no Nav1.6 mRNA could be detected in the ENS by ISH. The fact that adult myenteric neurons are endowed with only two TTX-sensitive α subunits, namely, Nav1.3 and Nav1.7, emphasizes the singularity of the ENS. Both these subunits, known to have slow-inactivation kinetics, are well adapted for generating action potentials from slow excitatory postsynaptic potentials, a mode of synaptic transmission that applies to all ENS neuron types. J. Comp. Neurol. 504:363-378, 2007. © 2007 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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