| Autor: |
Osborn, E. C., Wootton, I. D. P. |
| Zdroj: |
Journal of Clinical Pathology; Mar1964, Vol. 17 Issue 2, p156-159, 4p |
| Abstrakt: |
A shortened method of estimating serum bile acids is described, based on extraction and the measurement of fluorescence in sulphuric acid solution at 37°C. The cholate and dihydroxy bile acid fractions are determined separately by measurements made one hour and 24 hours after preparing the solution. About 1% of the serum cholesterol escapes separation and appears in the sulphuric acid solution; in its fluorescent properties it behaves in a manner almost identical with dihydroxy bile acids. As a result, the normal serum bile acids are overestimated by about 2 mg./100 ml. This effect is unimportant in cases of jaundice. In non-jaundiced cases with hypercholesterolaemia, the apparently raised serum bile acid levels are accounted for by the interference from cholesterol and there is no evidence that a raised serum cholesterol level is usually associated with raised serum bile acids. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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