A noninvasive [99mTc]DTPA SPECT/CT imaging methodology as a measure of lung permeability in a guinea pig model of COPD.

Autor: Wu, Yanjun, Kotzer, Charles, Makrogiannis, Sokratis, Logan, Gregory, Haley, Hyking, Barnette, Mary, Sarkar, Susanta, Kotzer, Charles J, Logan, Gregory A, Barnette, Mary S, Sarkar, Susanta K
Předmět:
Zdroj: Molecular Imaging & Biology; Oct2011, Vol. 13 Issue 5, p923-929, 7p
Abstrakt: Purpose: The purposes of this study are (1) to develop an efficient aerosol inhalation system for the delivery of [(99m)Tc]DTPA aerosol into guinea pig airways with high uniformity for measuring lung clearance using SPECT/CT imaging, as a measure of lung permeability, and (2) to use [(99m)Tc]DTPA studies in guinea pig model of chronic obstructive pulmonary disease (COPD) to determine its usefulness in studying pathogenesis of COPD.Procedure: We developed an aerosol delivery system and single-photon emission computed tomography (SPECT) imaging method to measure the pulmonary clearance rate in anesthetized guinea pigs. In this system, an 830-cc rebreather exposure chamber was filled with oxygen immediately before a 5 min [(99m)Tc]DTPA (4-5 mCi/mL) aerosol exposure. The rebreather included a top mounted AeroNeb micro pump nebulizer, a nose-only exposure tube, and rear evacuation port. An 830-cc rebreather exposure chamber was filled with oxygen immediately before 5 min [(99m)Tc]DTPA (4 ∼ 5 mCi/mL) aerosol exposure. One control and one cigarette smoking group were studied.Results: Images showed high and uniform lung deposition and the mean clearance rate was increased by 37% in smoking group.Conclusions: The combined SPECT/CT imaging method developed here can be used for serial evaluation of lung function and its response to drug therapy in guinea pig model of COPD. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz: