| Autor: |
Gordeev, Alexander A., Samatov, Timur R., Chetverina, Helena V., Chetverin, Alexander B. |
| Zdroj: |
Biotechnology & Bioengineering; Nov2011, Vol. 108 Issue 11, p2682-2690, 9p |
| Abstrakt: |
We present a method for generating gel-based unordered 2D arrays of bacterial cells of a very high density, up to 105 cells per mm2. Bacteria in a suspension are focused into a thin layer when the suspension and a dry gel matrix penetrate each other. Formation of a second gel from gel-forming components contained in the suspension results in immobilization of the cells. The immobilized cells stay alive and can repeatedly divide to produce microcolonies. The method provides for high-throughput screening and massively parallel analysis of individual cells in large populations, as well as for rapid isolation of rare clones. Biotechnol. Bioeng. 2011;108: 2682-2690. © 2011 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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