| Autor: |
Kourí, Vivian, Martínez, Pedro A., Blanco, Orestes, Capó, Virginia, Rodríguez, María E, Dovigny, María del C, Cardellá, Lidia, Gala, Angela, Jiménez, Narciso A., Correa, Consuelo, Alemán, Yoan, Pérez, Lissette, Álvarez, Alina, Hengge, Ulrich |
| Předmět: |
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| Zdroj: |
Herpesviridae; 2010, Vol. 1 Issue 1, p1-7, 7p, 2 Charts, 2 Graphs |
| Abstrakt: |
In Cuba, previous reports have shown an increase of epidemic KS, reaching a total of 120 cases by the end of 2007, despite the use of HAART. To evaluate and compare the role of human herpes virus 8 (HHV-8) viral loads in different compartments of AIDS-related Kaposi's sarcoma (AIDS-KS) patients real-time polymerase chain reaction (RT-PCR) was used to determine the genome copy number of HHV-8 in plasma, saliva, tissue and peripheral blood mononuclear cells (PBMC) of 49 AIDS-KS patients. Overall, 98% of AIDS-KS patients harbored detectable HHV-8. HHV-8 could be detected in 91.6% of KS tissue lesions showing the highest viral load (median log = 3.14 copies/ 100 ng DNA) followed by saliva and PBMC which were positive in 78%, and 69.2%; respectively. In contrast, HHV-8 was detected in only 37% of plasma samples, which also showed lower viral loads. Men who had sex with men (MSM) were more likely to have three-times higher HHV-8 genome copies in KS lesions when compared with tissues from heterosexuals individuals (OR 3; 95% CI 1.1 to 12.5). These results emphasize the systemic nature of HHV- 8-infection and demonstrate the possible role of saliva in HHV-8 transmission among MSM. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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