| Autor: |
Tsuchiya, Hiroyuki, Harashima, Hideyoshi, Kamiya, Hiroyuki |
| Zdroj: |
Journal of Gene Medicine; Apr2005, Vol. 7 Issue 4, p486-493, 8p |
| Abstrakt: |
Background The correction of a mutated gene by the small fragment homologous replacement (SFHR) method is a highly attractive approach for gene therapy. However, the current SFHR method with a heat-denatured double-stranded PCR fragment yielded a low correction efficiency. Methods Single-stranded (ss) DNA fragments were prepared from ss phagemid DNA and tested in a gene correction assay with an inactivated Hyg-EGFP fusion gene, as a model target. Results A 606-nt sense, ss DNA fragment dramatically (12-fold) improved the gene correction efficiency, although the antisense strand showed only minimal correction efficiency. Conclusions These results suggest that the use of a sense, single-stranded DNA fragment is useful in the SFHR method for the correction of mutated genes. Copyright © 2004 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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