| Autor: |
Nirungsan, Katthaleeya, Thongnopnua, Phensri |
| Zdroj: |
Biomedical Chromatography; Aug2006, Vol. 20 Issue 8, p774-781, 8p |
| Abstrakt: |
A simple and rapid reversed-phase high-performance liquid chromatographic method was developed and validated for the determination of endogenous α-tocopherol in human plasma. Following addition of α-tocopheryl acetate as the internal standard, the plasma was deproteinized using acetonitrile and isopropanol mixture prior to HPLC analysis. Methanol was used as the mobile phase and the effluent was quantitated at 292 nm. By this developed method, the concentrations of α-tocopherol were linearly related to their responses in the range of 0.8-30 µg/mL. The relative standard deviations intra-day and inter-day for α-tocopherol in plasma were less than 10%. The percentage of bias was within ±4%, which confirmed the accuracy of the method. The method has been successfully applied for determining endogenous α-tocopherol in healthy Thai male volunteers. Copyright © 2005 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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