| Autor: |
Zhou, Ai-min, Wu, Duo, Che, Dai-di, Liu, Shen-kui, Yang, Chuan-ping, Wang, Jin-gang |
| Zdroj: |
Journal of Forestry Research (1007662X); Sep2011, Vol. 22 Issue 3, p361-366, 6p |
| Abstrakt: |
Five different isoforms ( IrlVHA-c1- c5) of V-ATPase subunit c ( VHA-c) were cloned from a Japanese iris ( Iris lactea Pall. var. chinensis Fisch. Koidz) cDNA library using degenerate primers PCR and the 5′-RACE technique. The sequence analysis showed the open reading frame (ORF) of the IrlVHA-c1- c5 to be 495 bp, corresponding to a protein of 164 amino acids. Among the five isoforms, IrlVHA-c1 and IrlVHA-c2 are completely homologous. The IrlVHA-c protein is localized at the vacuolar membrane as indicated by a green fluorescent protein (GFP) marker. Its over-expression in yeast could enhance yeast tolerance to NaCl stress. These results show that there are at least five genes encoding different isoforms of IrlVHA-c in Japanese iris and IrlVHA-c is important for the function of V-ATPase. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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