| Autor: |
Xin, Lijing, Mlynárik, Vladimír, Lanz, Bernard, Frenkel, Hanne, Gruetter, Rolf |
| Zdroj: |
Magnetic Resonance in Medicine; Aug2010, Vol. 64 Issue 2, p334-340, 7p |
| Abstrakt: |
Full signal intensity 1H-[13C] NMR spectroscopy, combining a preceding 13C-editing block based on an inversion BISEP (B1-insensitive spectral editing pulse) with a spin-echo coherence-based localization, was developed and implemented at 14.1 T. 13C editing of the proposed scheme was achieved by turning on and off the 13C adiabatic full passage in the 13C-editing block to prepare inverted and noninverted 13C-coupled 1H coherences along the longitudinal axis prior to localization. The novel 1H-[13C] NMR approach was applied in vivo under infusion of the glia-specific substrate [2-13C] acetate. Besides a ∼50% improvement in sensitivity, spectral dispersion was enhanced at 14.1 T, especially for J-coupled metabolites such as glutamate and glutamine. A more distinct spectral structure at 1.9-2.2 ppm(parts per million) was observed, e.g., glutamate C3 showed a doublet pattern in both simulated 1H spectrum and in vivo 13C-edited 1H NMR spectra. Besides 13C time courses of glutamate C4 and glutamine C4, the time courses of glutamate C3 and glutamine C3 obtained by 1H-[13C] NMR spectroscopy were reported for the first time. Such capability should greatly improve the ability to study neuron-glial metabolism using 1H-observed 13C-edited NMR spectroscopy. Magn Reson Med, 2010. © 2010 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text