Comparison of Purification Procedures for p-Conglycinin and the Effect of Toasting.

Autor: WINTERS, D. D., CUPPETT, S. L., ZEECE, M. G.
Předmět:
Zdroj: Journal of Food Science (Wiley-Blackwell); Jan1990, Vol. 55 Issue 1, p283-284, 2p
Abstrakt: BSTRACT A comparison of the effectiveness of two procedures, ammonium sulfate fractionation and concanavalin A affinity (Con A) chromatography, for the purification of β-conglycinin from soy flour was made. In addition, the effect of toasting on the effectiveness of these two procedures for purifying β-conglycinin was studied. Results indicated that Con A affinity chromatography was less effective than the ammonium sulfate fractionation for the purification of β-conglycinin. The ammonium sulfate fractionation produced nearly homogeneous β-conglycinin; however, the yield from this procedure was very low (4.1%). Toasting the soy flour decreased the purification effectiveness of both procedures. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index