| Autor: |
Hua Ping Zhu, Mai Xin Lu, Zhang Han Huang, Feng Ying Gao, Dong Mei Ma, Li Zhou, Jian Fang Gui |
| Předmět: |
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| Zdroj: |
Aquaculture Research; Jul2011, Vol. 42 Issue 8, p1178-1185, 8p |
| Abstrakt: |
Chromosomal karyotypes of Oreochromis mossambicus and O. urolepis hornorum and their hybrid were analysed by means of Cot-1 DNA bandings through ￡uorescence in situ hybridization (FISH). To identify all chromosomes, Cot-1DNA ^which contains highly and moderately repetitive DNA ^ was extracted from genomic DNA, labelled as a probe with Dig-11-dUTP, and in situ hybridized to spreads of mitotic chromosomes of the three samples. The hybridized signals were detected by means of Cy3-conjugated antidigoxigenin. The FISH results indicated that the three samples had the same diploid number (2n544) of chromosomes. Speci￠c ￡uorescence signal bands were detected on all individual chromosome pairs. On the basis of Cot-1 DNA FISH banding patterns and chromosome morphology, the karyotypes of the three samples have been constructed; no remarkable di!erences were detected between the karyotypes of these species using this method. These results ^which are similar to those reported previously, with respect to chromosome number, morphology and Cot-1DNA FISH patterns ^ suggest chromosomal stasis during speciation and hybridization of tilapia (Oreochromis, Cichlidae). Such a molecular cytogenetic procedure, if used in conjunctionwith other genomic research methods, could facilitate the study of genomic structure and be adapted for chromosome studies of other animal species. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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