Diagnosis of Equid herpesviruses −1 and −4 by polymerase chain reaction.

Autor: SHARMA, P. C., CULLINANE, A. A., ONIONS, D. E., NICOLSON, L.
Zdroj: Equine Veterinary Journal; Jan1992, Vol. 24 Issue 1, p20-25, 6p
Abstrakt: Summary The polymerase chain reaction (PCR) is a sensitive technique used to detect DNA of viral pathogens. We have applied the technique to the detection of Equid herpesviruses-1 and −4 (EHV-1 and EHV-4) DNA within nasopharyngeal swab samples from horses. Ninety-eight samples from suspected field cases and in-contact horses were analysed. The assays were conducted blind and later decoded and compared with virus isolation data. Our results indicate that PCR is a sensitive and rapid technique for the diagnosis of EHV-1 and EHV-4 infection. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index