| Autor: |
Cendron, Delphine, Ingoure, Sophie, Martino, Angelo, Casetti, Rita, Horand, Françoise, Romagné, François, Sicard, Hélène, Fournié, Jean-Jacques, Poccia, Fabrizio |
| Zdroj: |
European Journal of Immunology; Feb2007, Vol. 37 Issue 2, p549-565, 17p |
| Abstrakt: |
Phosphoantigens are mycobacterial non-peptide antigens that might enhance the immunogenicity of current subunit candidate vaccines for tuberculosis. However, their testing requires monkeys, the only animal models suitable for γδ T cell responses to mycobacteria. Thus here, the immunogenicity of 6-kDa early secretory antigenic target-mycolyl transferase complex antigen 85B (ESAT-6-Ag85B) (H-1 hybrid) fusion protein associated or not to a synthetic phosphoantigen was compared by a prime-boost regimen of two groups of eight cynomolgus. Although phosphoantigen activated immediately a strong release of systemic Th1 cytokines (IL-2, IL-6, IFN-γ, TNF-α), it further anergized blood γδ T lymphocytes selectively. By contrast, the hybrid H-1 induced only memory αβ T cell responses, regardless of phosphoantigen. These latter essentially comprised cytotoxic T lymphocytes specific for Ag85B (on average + 430 cells/million PBMC) and few IFN-γ-secreting cells (+ 40 cells/million PBMC, equally specific for ESAT-6 and for Ag85B). Hence, in macaques, a prime-boost with the H-1/phosphoantigen subunit combination induces two waves of immune responses, successively by γδ T and αβ T lymphocytes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text