| Autor: |
Toga, Hirohisa, Tobe, Takeyasu, Ueda, Yoshimichi, Yang, Guan-Hu, Osanai, Kazuhiro, Ishigaki, Masanobu, Okazaki, Hiroshi, Katsuda, Shogo, Takahashi, Keiji, Ohya, Nobuo |
| Předmět: |
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| Zdroj: |
Experimental Lung Research; Sep2001, Vol. 27 Issue 6, p485-504, 20p |
| Abstrakt: |
Alveolar type II cells (type II cells)play a crucial role in the progression and repair of lung inflammation and injury. We investigated whether inducible nitric oxide synthase (iNOS) was expressed and nuclear factor-κB (NF-κB)was activated in type IIcells in lung injury. After injecting lipopolysaccharide (LPS)or saline in the rat, the lungs were excised and type II cells were isolated. iNOS and its mRNAwere expressed both in lung tissue and isolated type II cells in response to LPS. The lungs from saline-treated rats showed only minimal expression ofiNOS. Electrophoretic mobility shift assay revealed that expression of NF-κB in the nuclear extracts was augmented by LPS, and p50/NF-κB was expressed in type II cells in LPS-treated rats. Intraperitoneal dexamethasone almost completely inhibited the iNOS expression and attenuated the activation ofNF- Bin the LPS-treated lung. These findings suggest that type II cells can be a source of NO production in lung injury, and that the effects ofcorticosteroids may be in part through inhibition of both iNOS expression and NF-κB activation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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