Production and characterization of the monoclonal antibodies to Bacillus anthracis protective antigen.

Autor:	Rudenko, N. V., Abbasova, S. G., Grishin, E. V.
Předmět:	MONOCLONAL antibodies BACILLUS anthracis ANTIGENS ENZYME-linked immunosorbent assay IMMUNOGLOBULINS
Zdroj:	Russian Journal of Bioorganic Chemistry; May2011, Vol. 37 Issue 3, p316-321, 6p, 1 Black and White Photograph, 2 Charts, 1 Graph
Abstrakt:	nthrax, a cosmopolitan acute infectious disease affecting animals and humans, is a category A bioterrorism threat agent because of the high resistance of Bacillus anthracis spores to adverse environmental factors and their easy production. In this work, we have obtained a representative panel of 20 monoclonal antibodies to the anthrax protective antigen, a key component of the pathogenic exotoxin. A sandwich ELISA for quantification of the protective antigen using these antibodies was developed. Six pairs of monoclonal antibodies are able to detect the protective antigen in blood serum with a detection limit of 1 ng/ml. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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