| Autor: |
Chuck, Roy S., Behrens, Ashley, Wellik, Sarah, Liaw, Leacky L. H., Dolorico, Arlene M. T., Sweet, Paula, Chao, Lawrence C., Osann, Kathryn E., McDonnell, Peter J., Berns, Michael W. |
| Předmět: |
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| Zdroj: |
Archives of Ophthalmology; Nov2001, Vol. 119 Issue 11, p1637, 6p |
| Abstrakt: |
Objective: To describe the epithelial healing rates observed in freshly cultured rabbit corneas chemically burned with high-concentration hydrochloric acid (HCl) and sodium hydroxide (NaOH) and subsequently treated with phototherapeutic keratectomy (PTK). Methods: We obtained 126 fresh corneoscleral rims from cadaveric New Zealand white rabbits. Each cornea was exposed to 4-mm cellulose sponges soaked in a solution of topical 0.9% isotonic sodium chloride solution, 2M HCl, or 0.5M NaOH. A transepithelial PTK (6-mm zone; 100-pm ablation depth) was then performed using the excimer laser (150-mJ/cm² energy pulse; 20 nanosecond duration; and 10-Hz frequency). Corneas were placed in tissue culture, and 1 cornea from each group was taken out of culture each day after treatment. Re-epithelialization was monitored by means of fluorescein staining, slitlamp photography, and histopathological analysis. Results: Corneas treated with HCl and NaOH exhibited immediate epithelial defects that slowly healed over time. In PTK-treated corneas, the re-epithelialization rate was accelerated compared with that of controls (P = .003 for the HCl group, and P<.001 for the NaOH group). The new epithelial layers were smoother in PTK-treated corneas, as confirmed by results of histopathological analysis. Conclusion: Corneal damage caused by HCl and NaOH may be modulated in vitro by PTK in this rabbit model. Clinical Relevance: After corneal chemical damage, 193-nm excimer laser PTK accelerates epithelial wound healing. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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