| Abstrakt: |
sensitive and selective analytical method for the quantification of pregabalin, sildenafil and the active desmethyl metabolite of sildenafil (UK-103320) has been developed. The method can simultaneously quantify the three analytes within the expected in vivo concentration ranges using 50 μL of rat plasma. It utilises solid-phase extraction followed by high performance liquid chromatography coupled with tandem mass spectrometry. Quantitation in rat plasma demonstrated good accuracy and precision over the following dynamic ranges for each analyte: pregabalin (70-10,000 ng mL), sildenafil (1-2,000 ng mL) and UK-103320 (1-2,000 ng mL). For each analyte, the following lower limits of quantitation were obtained: 70 ng mL for pregabalin and 1 ng mL for sildenafil and UK-103320, respectively. The method was successfully used to analyse plasma samples from rats when pregabalin and sildenafil were administered in combination. [ABSTRACT FROM AUTHOR] |
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